Environmental polycyclic aromatic hydrocarbon (PAH) exposure

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Environmental polycyclic aromatic hydrocarbon (PAH) exposure

Postby Wilberforce » Sun Dec 25, 2011 9:44 pm

Environmental polycyclic aromatic hydrocarbon (PAH) exposure and DNA damage in Mexican children

Marco Sánchez-Guerraa, E-mail The Corresponding Author, Nadia Pelallo-Martínezb, E-mail The Corresponding Author, Fernando Díaz-Barrigab, E-mail The Corresponding Author, Stephen J. Rothenberga, c, E-mail The Corresponding Author, Leticia Hernández-Cadenac, E-mail The Corresponding Author, Sylvain Faugerond, E-mail The Corresponding Author, Luis F. Oropeza-Hernándeze, E-mail The Corresponding Author, Margarita Guaderrama-Díaza, E-mail The Corresponding Author, Betzabet Quintanilla-Vegaa, Corresponding Author Contact Information
http://www.sciencedirect.com/science/ar ... 1811003597

Received 22 September 2011; revised 2 December 2011; Accepted 8 December 2011. Available online 17 December 2011.
Abstract

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous pollutants presenting a public health risk, particularly to children, a vulnerable population. PAHs have genotoxic and carcinogenic properties, which depend on their metabolism. Many enzymes involved in PAH metabolism, including CYP1A1, CYP1B1, GSTM and GSTT are polymorphic, which may modulate the activation/deactivation of these compounds. We evaluated PAH exposure and DNA damage in children living in the vicinity of the main petrochemical complex located in the Gulf of Mexico, and explored the modulation by genetic polymorphisms of PAH excretion and related DNA damage. The participants (n = 82) were children aged 6–10 y attending schools near the industrial area. Urinary 1-hydroxypyrene (1-OHP; a biomarker of PAH exposure) was determined by reverse-phase-HPLC; DNA damage by the comet assay (Olive Tail Moment (OTM) parameter); CYP1A1*2C and CYP1B1*3 polymorphisms by real time-PCR; and GSTM1*0 and GSTT1*0 by multiplex PCR. The median value of 1-OHP was 0.37 μmol/mol creatinine; 59% of children had higher 1-OHP concentrations than those reported in environmentally exposed adults (0.24 μmol/mol creatinine). A stratified analysis showed increased DNA damage in children with 1-OHP concentrations greater than the median value. We observed higher 1-OHP concentrations in children with CYP1A1*2C or GSTM1*0 polymorphisms, and a positive influence of CYP1A1*2C on OTM values in children with the highest PAH exposure. The data indicate that children living in the surroundings of petrochemical industrial areas are exposed to high PAH levels, contributing to DNA damage and suggesting an increased health risk; furthermore, data suggest that polymorphisms affecting activation enzymes may modulate PAH metabolism and toxicity.
Highlights

► Children from the studied industrial site are exposed to high PAH level. ► Environmental high PAH exposure is related to DNA damage in children. ► Environmental PAH exposure limit has to be established. ► Alteration on 1-OHP excretion by genetic polymorphisms may also modify PAH toxicity.

Abbreviations: CYP, cytochrome P450; GST, glutathione-S-transferase; 1-OHP, 1-hydroxypyrene; PAH, polycyclic aromatic hydrocarbon; UGT, UDP-glucuronosyl transferase

Keywords: PAH metabolism; 1-Hydroxypyrene; DNA damage; Environmental exposures; Genetic polymorphisms
Article Outline

1. Introduction
2. Materials and methods
2.1. Study population
2.2. Sample collection and analysis
2.3. DNA extraction and genotyping
2.4. Comet assay
2.5. Statistical analyses
3. Results
3.1. Children's characteristics and their influence on urinary 1-OHP concentrations
3.2. DNA damage
3.3. Genetic polymorphisms
3.4. Influence of genetic polymorphisms of PAH metabolizing enzymes on the biomarker of exposure (1-OHP)
3.5. Influence of genetic polymorphisms of PAH metabolizing enzymes on DNA damage
4. Discussion
5. Conclusions
Conflict of interest statement
Financial source
Acknowledgments
References


Corresponding Author Contact InformationCorresponding author at: Ave. IPN 2508, Col. Zacatenco, Mexico City 07360, Mexico. Tel.: +52 55 57473800x5446; fax: +52 55 57473395.
• The Surgeon General has determined that there is no safe level of exposure to ambient smoke!

• If you smell even a subtle odor of smoke, you are being exposed to poisonous and carcinogenic chemical compounds!

• Even a brief exposure to smoke raises blood pressure, (no matter what your state of health) and can cause blood clotting, stroke, or heart attack in vulnerable people. Even children experience elevated blood pressure when exposed to smoke!

• Since smoke drastically weakens the lungs' immune system, avoiding smoke is one of the best ways to prevent colds, flu, bronchitis, or risk of an even more serious respiratory illness, such as pneumonia or tuberculosis!
Does your child have the flu? Chances are they have been exposed to ambient smoke!
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