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Differential Effects of Particulate Matter

PostPosted: Wed Feb 25, 2015 10:13 am
by Wilberforce
Differential Effects of Particulate Matter Upwind and Downwind of an Urban Freeway in an Allergic Mouse Model
McGee et al
Environ. Sci. Technol., Just Accepted Manuscript

neutrophils and eosinophils are a type of white blood cell

Near-road exposures to air pollutants have been associated with decreased lung function and other adverse health effects in susceptible populations. This study was designed to investigate whether different types of near-road particulate matter (PM) contribute to exacerbation of allergic asthma. Samples of upwind and downwind coarse, fine, and ultrafine PM were collected using a wind direction-actuated ChemVol sampler at a single site 100 meters from Interstate-96 in Detroit, MI during winter 2010/2011. Upwind PM was enriched in crustal and wood combustion sources while downwind PM was dominated by traffic sources. Control and ovalbumin (OVA)-sensitized BALB/cJ mice were exposed via oropharyngeal (OP) aspiration to 20 or 100 µg of each PM sample two hours prior to OP challenge with OVA. In OVA-allergic mice, 100 µg downwind coarse PM caused greater increases than downwind fine/ultrafine PM in bronchoalveolar lavage neutrophils, eosinophils, and lactate dehydrogenase. Upwind fine PM (100 µg) produced greater increases in neutrophils and eosinophils compared to other upwind size fractions. Cytokine (IL-5) levels in BAL fluid also increased markedly following 100 µg downwind coarse and downwind ultrafine PM exposures. These findings indicate coarse PM downwind and fine PM upwind of an interstate highway promote inflammation in allergic mice.